贺新春,杜何为,黄敏

• 1:长江大学生命科学学院

摘要(Abstract)：

在植物应对环境胁迫的反应中，钙调蛋白（CaM）发挥着重要作用。基于玉米（Zea mays L.）自交系B73的转录组数据克隆了一个在干旱胁迫后根系高水平表达的CaM基因，并对其进行了生物信息学分析。结果表明，ZmCaM1基因的编码序列大小为450 bp，编码149个氨基酸。ZmCaM1蛋白不含信号肽且具有亲水性特征。在EF-hand结构域上，ZmCaM1蛋白与高粱（Sorghum bicolor L.）和水稻（Oryza sativa L.）等植物中的CaM蛋白高度保守，呈现相似的二级结构和三级结构。此外，ZmCaM1启动子序列中发现了多个与激素和非生物胁迫相关的顺式作用元件。根据蛋白互作预测和酵母双杂交结果，ZmCaM1蛋白与DNA修复蛋白RAD4互作。通过对原核表达蛋白诱导条件的优化，GST-ZmCaM1在0.1 mmol/L IPTG条件下的表达效果最佳，并经凝胶亲和层析纯化得到了GST-ZmCaM1蛋白。表达分析结果显示，ZmCaM1基因在玉米出苗期的根和叶中表达水平较高。干旱胁迫后该基因在根和叶中上调表达，涝渍胁迫后该基因在根和叶中下调表达，进一步验证了ZmCaM1基因与玉米生长发育以及非生物胁迫响应之间的联系。

关键词(KeyWords)： 玉米;钙调蛋白;非生物胁迫;生物信息学;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31771801);; 湖北省自然科学基金面上项目(2022CFB183);湖北省自然科学基金创新群体项目(2022CFA030)

作者(Author): 贺新春,杜何为,黄敏

DOI: 10.16035/j.issn.1001-7283.2025.04.003

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